Bioactive agent for prevention & control of Mycobacteriumavium subspecies paratuberculosis

Background and Aim : Mycobacterium avium subspecies paratuberculosis (MAP) is thecausative agent of paratuberculosis, Johne's disease or (JD) in cattle & Crohn's disease (CD) inhuman. Currently there are not any successful treatments for MAP so development of an effectivemethod for prevention will be yielded significant results. Pathogen-specific IgY secretoryantibodies can be produced and purified with high efficiency from egg yolk by inexpensive andreliable methods and can be used for complementary prevention and treatment of JD or CD disease.The aim of this study was to investigated the effects of IgY on growth of MAP growth in vitro.Methods : Specific IgY was produced by immunizing hens with formalin-killed MAP strains III& V. The Specific IgY against MAP was purified from the yolks using PEG ۶۰۰۰. IgY wasidentified by SDS-PAGE and its activity against MAP was measured by ELISA. The proteinconcentration of the purified IgY was determined using Kjeldahl method. The growth inhibitionassays were also conducted to monitor the growth changes.Results : The ELISA result indicated the specific activity of purified IgY to the MAP. The growthinhibition assay revealed that the lowest concentration of anti-MAP IgY was ۲μg/ml. The possiblemechanism of bacteriostatic function of IgY is due to adherence of the immunoglobulin to exposedfactors on the surface of MAP, resulted in alteration of cellular signaling. IgYs could also cause ofMAP agglutination , leading to their immobilization, therefore it interferes with the growth.Conclusion : Anti-MAP IgYs may play as a promising new preventative bioagent for entericpathogen infections.

کلیدواژه ها:

inhibition of bacterial growth ، chicken egg yolk antibody ، IgY ، ELISA ، Mycobacterium avium subspecies paratuberculosis