Molecular detection of lsa۲۱ gene encoding an adhesionprotein of pathogenic leptospiral serovars

Background and Aim : Leptospirosis is an allinclusive broad zoonosis which caused bypathogenic leptosires. To get into molecular pathogenicity of leptospirosis, it is essential to studythe genetic characteristics of genes encoding outer membrane proteins. The Lsa۲۱ is one of theleptospiral proteins which has extracellular matrix-binding properties. It is an immunogenicprotein which is only present in pathogenic serovars, Therefore it is a suitable candidate to be usedin an effective vaccine preparation and diagnostic methods. Hence detection of pathogenicleptospires from non-pathogenic serovars with PCR assay was conducted on lsa۲۱ gene.Methods : Leptospiral serovars required in this study were obtained from microbial collection ofRazi institute, karaj, Iran. The leptospiral genomic DNA was extracted by Phenol-Chloroformextraction method. The gene was amplified by PCR from pathogenic Leptospira serovars andsaprophyte serovars genomic DNA. Inorder to confirm the results, the number of PCR productscontaining the target gene were sequenced. The sequence analysis was performed using blastn andChromas.Results : The PCR of lsa۲۱ gene was a ۵۴۰ bp fragment which was observed in pathogenicserovars and not observed in non-pathogenic serovars. The basic sequence examination wascarried out to discover homologous target sequence of nucleotide from the distinctive databasesshowed high similarity among the leptospiral serovars and reference sequences submitted inGenBank.Conclusion : The result indicated that lsa۲۱ gene has high conservation between pathogenicleptospiral serovars. Molecular discovery of pathogenic leptospires based on lsa۲۱ gene can beutilized for laboratory diagnosis as a recombinant antigen and planning subunit vaccines ofleptospirosis.

کلیدواژه ها:

Leptospirosis ، Rice-Field Fever ، lsa۲۱ gene ، molecular detection ، LeptospiraInfections