Distribution of virulence genes among Staphylococcussaprophyticus isolated from patients with UTI

Background and Aim : Urinary tract infection (UTI) is one of the most common bacterialinfections caused by gram-positive and gram-negative factors. Staphylococcus saprophyticus is acommon gram-positive agent that causes uncomplicated UTI in women.The present study aimedto determine detect genes encoding surface proteins Aas,SdrI,Ssp,Uaf that play a role in theinfections.Methods : This study was financially supported by a grant from Golestan University of MedicalSciences, Gorgan, Iran. (IR.GOUMS.REC.۱۴۰۱.۰۶۱).A total of ۳۵ S. saprophyticus isolates wereobtained from female patients with UTI. All the strains were identified by PCR for ۱۶S rRNAGene.For the amplification of the virulence genes the primers were designed in this study.Results : The distribution of the virulence genes in Clinical Isolated was evaluated . Distributionof virulence genes encoding the surface proteins Aas,Ssp UafA were %۹۸ ,۹۲% and ۸۴%respectively. In contrast, the gene encoding SdrI was not detected in any of the strains ofS.saprophyticus.Conclusion : These results provide a new insight of the characteristics of S. saprophyticus strainsand it shows that all this surface proteins necessary to colonize the urinary tract.

کلیدواژه ها:

Staphylococcus saprophyticus ، virulence genes ، Urinary tract infection