Prevalence of blaTEM gene among Escherichia coli strainsisolated from Tabriz hospitals
محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 89
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شناسه ملی سند علمی:
MEDISM23_297
تاریخ نمایه سازی: 16 مهر 1401
چکیده مقاله:
Background and Aim : Escherichia coli (E.coli), which produces extended spectrum β-lactamases (ESBLs), has become a significant issue on a global scale in recent years. The TEMfamily includes the majority of β-lactamases. This study's primary goal was to determine howfrequently the blaTEM β-lactamase gene was found in E. coli isolates from Tabriz, Iran.Methods : A total of ۲۳۲ E. coli strains recovered from clinical samples in Tabriz hospitals werethe subject of this cross-sectional study. By using the disc diffusion approach, the antibioticsusceptibility pattern of each isolate was identified. After that by using a combined disk test in thepresence of cefotaxime and clavulanic acid, the ESBL-producing strains were identified. Usingthe PCR technique, the TEM gene's existence in ESBLs was evaluated.Results : The prevalence of ESBL-producing E. coli was ۳۳%, according to the combined disktestresults. ۲۷ percent of the ESBL-producing E. coli tested positive for the TEM gene. Tazocin(۹۱%) and aztreonam (۹۰%) had the highest rates of sensitivity, although they were resistant tocefuroxime (۵۷% ).Conclusion : Most ESBL-producing E. coli bacteria recovered from Tabriz hospitals share theTEM gene. Monitoring the ESBLs producing E. coli in hospitals requires ongoing surveillance.The therapy of ESBL infections is greatly hampered by resistance to β-lactam medicines.
کلیدواژه ها:
نویسندگان
Sana Falahi
BSc student of Laboratory Sciences, Sarab Faculty of Medical Sciences, Sarab, Iran
Mohaddese Ghafourifard
BSc student of Laboratory Sciences, Sarab Faculty of Medical Sciences, Sarab, Iran
Sepideh Asadi
BSc student of Laboratory Sciences, Sarab Faculty of Medical Sciences, Sarab, Iran
Morteza Akbari
Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz-Iran
Mehdi Marzi
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Fenerbahce University, Istanbul-Turkey
Ali Bahadori
Department of Medical Microbiology, Sarab Faculty of Medical Sciences, Sarab, Iran