Prevalence of blaTEM gene among Escherichia coli strainsisolated from Tabriz hospitals

Background and Aim : Escherichia coli (E.coli), which produces extended spectrum β-lactamases (ESBLs), has become a significant issue on a global scale in recent years. The TEMfamily includes the majority of β-lactamases. This study's primary goal was to determine howfrequently the blaTEM β-lactamase gene was found in E. coli isolates from Tabriz, Iran.Methods : A total of ۲۳۲ E. coli strains recovered from clinical samples in Tabriz hospitals werethe subject of this cross-sectional study. By using the disc diffusion approach, the antibioticsusceptibility pattern of each isolate was identified. After that by using a combined disk test in thepresence of cefotaxime and clavulanic acid, the ESBL-producing strains were identified. Usingthe PCR technique, the TEM gene's existence in ESBLs was evaluated.Results : The prevalence of ESBL-producing E. coli was ۳۳%, according to the combined disktestresults. ۲۷ percent of the ESBL-producing E. coli tested positive for the TEM gene. Tazocin(۹۱%) and aztreonam (۹۰%) had the highest rates of sensitivity, although they were resistant tocefuroxime (۵۷% ).Conclusion : Most ESBL-producing E. coli bacteria recovered from Tabriz hospitals share theTEM gene. Monitoring the ESBLs producing E. coli in hospitals requires ongoing surveillance.The therapy of ESBL infections is greatly hampered by resistance to β-lactam medicines.

کلیدواژه ها:

E-coli ، Extended-spectrum beta-lactamase (ESBL) ، TEM gene