Characterization of Antibacterial and Antioxidant Propertiesof Flowers of Ziziphora clinopodioides

Background and Aim : Plants with medicinal properties play an increasingly important role infood and pharmaceutical industries for their functions on disease prevention and treatment. Theaim of this project was to characterization the antibacterial and antioxidant properties ofmethanolic extract of flowers of Ziziphora clinopodioides (Lamiaceae).Methods : In this project, the flowers of Ziziphora clinopodioides were collected and methanolicextract prepared by microwave assisted extraction (MAE) method. Then, in vitro antioxidantactivity of methanolic extracts were assayed by ۱,۱-diphenyl-۲-picrylhydrazyl (DPPH۰) freeradical scavenging method of Blois. and antibacterial effect of Ziziphora clinopodioides extractswas evaluated against pathogenic bacteria associated with human infections viz. Staphylococcusaureus (PTCC ۱۱۱۳), and Shigella dysenteriae (PTCC ۱۱۸۸) by Disk diffusion test.Results : The antioxidant activity of the investigated methanolic extract of flowers of Ziziphoraclinopodioides was scavenging ability of DPPH۰ radical scavenging activity (۵۶.۶۱%). Whereas,the IC۵۰ of methanolic extract of flowers of Ziziphora clinopodioides for DPPH۰ assay was(۳۶.۷۲±۰.۲۳) mg/ml respectively. The methanolic extract of flowers of Ziziphora clinopodioidesshowed significant antibacterial activity against both (Gram positive) and (Gram negative)bacteria.Conclusion : Thus, the methanolic extract of flowers of Ziziphora clinopodioides would be helpfulfor the preparation of pharmaceutically useful drugs to destroy pathogenic microbes. Furthermore,in the present study the phytochemical evaluation of Ziziphora clinopodioides were found to be apowerful antioxidant, antibacterial agent and this study can be continued for their structuralelucidation and pharmacological activity.

کلیدواژه ها:

Ziziphora clinopodioides ، Pathogenic Microbes ، DPPH۰ ، Shigella dysenteriae ، Diskdiffusion ، Pharmacological Activity