Synergistic effect of zinc oxide nanoparticles and Buniumpersicum essential oil against clinical strains of Pseudomonas aeruginosa

Background and Aim : The increasing use of antibiotics against bacterial infections has led to anincrease in drug resistance and adverse side effects. Pseudomonas aeruginosa is an opportunisticpathogen that causes a wide variety of infections. The combined use of nanoparticles and herbalcompounds is a new approach to increase their effectiveness against drug-resistant bacteria. Thisstudy was conducted with the aim of investigating the synergistic effects of zinc oxidenanoparticles and Bunium persicum essential oil against clinical strains of P. aeruginosa.Methods : In this study, ۳۰ clinical strains of Pseudomonas aeruginosa were selected from theurine samples of patients referred to Milad Hospital. the antibiotic sensitivity of strains wasevaluated by the disk diffusion method. Zinc oxide nanoparticles were prepared and thecomposition analysis of Bunium persicum essential oil was performed. The minimum inhibitoryconcentration (MIC) of B. persicum essential oil and zinc oxide nanoparticles were investigatedby broth microdilution method and the synergistic effect of essential oil and nanoparticles wasdetermined by the checkerboard method.Results : The highest antibiotic resistance of Pseudomonas aeruginosa strains was reported to theantibiotic Ticarcillin (۴۸%) and the lowest resistance to the antibiotic ciprofloxacin (۱۴%). TheMIC of B. persicum essential oil was obtained between ۰.۵-۴ μl/ml, and the MIC of zinc oxidenanoparticles for the studied strain was between ۳۲-۱۲۸ μg/ml. The interaction of B. persicumessential oil and zinc oxide nanoparticles showed a synergistic effect against ۶۰% of P. aeruginosastrains.Conclusion : The findings showed that the combined use of zinc oxide nanoparticles and Buniumpersicum essential oil can be a promising option to control the emergence of multi-drug resistantbacteria.

کلیدواژه ها:

synergism ، antibiotic resistance ، Bunium persicum ، essential oil ، Pseudomonasaeruginosa.