Antibiofilm activity of Cinnamomum zeylanicum essential oilagainst clinical strains of Acinetobacter baumannii

Background and Aim : Acinetobacter baumannii is one of the most important causes of hospitalinfections worldwide. One of the mechanisms of antibacterial resistance of this bacterium is bybiofilm formation. Considering the role of biofilms in the pathogenicity of bacteria, it seemsnecessary to find new treatment methods to inhibit the formation of biofilm by bacteria. One ofthe proposed solutions to deal with bacterial biofilm is the use of plant essential oils. The aim ofthis study is to investigate the antibiofilm activity of Cinnamomum zeylanicum essential oil againstclinical strains of A. baumannii.Methods : In this study, ۳۰ clinical strains of A. baumannii were selected from the urine samplesof patients referred to Milad Hospital and their ability to form biofilm based on binding topolymeric surfaces was evaluated. The minimum inhibitory concentration (MIC) of C. zeylanicumessential oil was determined by broth microdilution method against ۵ strains of A. baumannii withstrong biofilm, and the biofilm inhibition effect of the strains was evaluated in the presence of C.zeylanicum essential oil in concentrations of ۱.۲ MIC, MIC and ۲ MIC by calculating CUT OFF.Results : According to the results, out of ۳۰ strains of A. baumannii, ۲۴ strains (۸۰%) formed astrong biofilm. MIC of C. zeylanicum essential oil on A. baumannii strains with strong biofilmwas obtained between ۰.۵-۱ ?l/ml. All investigated strains formed a negative biofilm after theeffect of the essential oil in all its concentrations.Conclusion : The use of medicinal plant compounds, including cinnamon essential oil, can be oneof the effective strategies to deal with bacterial biofilm and reduce their resistance.

کلیدواژه ها:

biofilm ، antibiotic resistance ، essential oil ، Cinnamomum zeylanicum ، Acinetobacterbaumannii.