Isolation and identification of Burkholder cepacia fromrespiratory secretions of cystic fibrosis patients in East Azerbaijan, Iran

Background and Aim : Burkholderia cepacia complex (BCC) is among the main factors ofsecondary infections in cystic fibrosis (CF) patients. Infection by these organisms has beenreported worldwide, being an organism with high drug resistance in hospital-acquired infections.Despite many global advances in Burkholderia infection epidemics, BBCS still causes manydeaths in CF patients. This study investigates B. cepacia isolation and identification by culturingand selecting a proper molecular method and exact setting up an efficient, rapid, and specificELISA-based method.Methods : : Periodic ۳-month tests, including culturing and biochemical tests, were conducted inEast Azerbaijan Province (Iran) to detect secondary infections in CF patients from ۲۲ September۲۰۲۰ to ۲۱ June ۲۰۲۱. Swab samples of secretions were simultaneously taken from CF patients’sputum for culturing and the PCR test.Results : BBC was not isolated from ۱۰۰ sputum samples in culturing and biochemical tests. Ofall tested samples, five samples were diagnosed as BCC infected samples by the moleculardiagnostic test.Conclusion : Despite the widespread application of selective media and biochemical properties,BBC cannot be reliably identified even by commercial diagnostic systems. However, B. cepaciawas identified with high precision in this study by selecting a proper molecular method. It alsoseems that the use of the ELISA technique with native species is among the basic variables insetting up an efficient, rapid and specific method.

کلیدواژه ها:

Burkholderia cepacia complex ، Cystic Fibrosis ، PCR ، ELISA