Molecular detection of class I integron and its gene cassette toantibiotics in Klebsiella pneumoniae strains

Background and Aim : Integrons are one of the mobile genetic elements that can carry resistancegenes to different antibiotics. Meanwhile, the role of class I integron is important in creating andtransmitting antibiotic resistance. The purpose of this study is to isolate Escherichia coli strains,molecular investigation of class I integron and gene cassettes and determine antibiotic resistanceand sensitivity.Methods : This study was conducted on ۱۰۰ samples of Klebsiella pneumoniae isolated fromKhorramabad hospitals. After sampling and culture on specific media and DNA extraction, thepresence of class I integron gene and aadB cassette was done by PCR method. Antibioticsensitivity and resistance test was also done by disk diffusion methodResults : After examining ۱۰۰ strains, ۶۰ samples were resistant to all antibiotics and ۷۸ sampleshad multiple antibiotic resistance. The highest resistance was related to ticarcillin and cefepimeantibiotics, the lowest resistance was related to gentamicin and amikacin antibiotics. Out of ۱۰۰samples of Klebsiella pneumoniae, ۴۸ samples had int۱ gene and also out of ۳۴ positive integronsamples, ۲۳ samples had aadB cassette.Conclusion : In this study, according to the significant statistics of the presence of class I integronand the gene cassette inserted in it in Klebsiella pneumoniae isolates and its relationship with thepattern of multiple drug resistance, it can be concluded that these elements can play an importantrole in the creation and transmission of antibiotic resistance. have a biotic

کلیدواژه ها:

Class I integron ، antibiotic resistance ، Klebsiella pneumoniae ، gene cassette