The application of the loop-mediated isothermal amplificationmethod for rapid detection of methicillin-resistant Staphylococcusaureus

سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 102

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

MEDISM23_186

تاریخ نمایه سازی: 16 مهر 1401

چکیده مقاله:

Background and Aim : Methicillin-resistant Staphylococcus aureus (MRSA) is an importantproblem associated with significant mortality and morbidity and is well known as a predominantbacterial pathogen. The aim of this study was to identify MRSA strains. In this study (June ۲۰۱۸to June ۲۰۱۹) isolates of S. aureus were obtained from patients referred to teaching hospitals ofAhvaz, Iran.Methods : All isolates were confirmed by conventional microbiological methods. In the following,antimicrobial susceptibility testing (AST), MRSA screening, PCR detection of MRSA, and LAMPassay were performed.Results : Out of a total of ۱۵۶ staphylococcal isolates, ۱۲۶ isolates were identified as MRSA.Seventy-two (۵۷.۱%) MRSA isolates were recovered from the wound. All MRSA isolates weresensitive to vancomycin, linezolid, teicoplanin, quinupristin-dalfopristin, and tigecycline. Theresults of LAMP showed ۱۰۰% agreement with PCR. The sensitivity and specificity of the LAMPassays for the mecA genes were ۱۰۰% and ۱۰۰%, respectively.Conclusion : The LAMP assay is a rapid and simple method for the identification of MRSA.Because of its performance without the need for specific instrumentation, this method can be easilyemployed in medical centers for the detection of mecA.

کلیدواژه ها:

نویسندگان

Fatemeh Shahi

Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Azar Dokht Khosravi

Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Saeed Khoshnood

Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran

Effat Abbasi Montazeri

Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Melika Moradi

Department of Microbiology, School of Medicine, Abadan University of Medical Sciences, Abadan, Iran

Nabi Jomehzadeh

Department of Microbiology, School of Medicine, Abadan University of Medical Sciences, Abadan, Iran