In vitro formulation of Solenanthus circinnatus leavesmethanolic extract and its antibacterial and antifungal inhibitoryactivities

Background and Aim : There is a global emergence of multiple drug-resistant (MDR)microorganisms because of the overuse of antibiotics. The emergence of these bacteria reduces theeffectiveness of current antibiotic therapy, causing thousands of deaths. As a result, new alternativeantimicrobials are urgently required to address the issue of MDR microorganisms. In this study,the antibacterial activity of methanolic extract of Solenanthus circinnatus (S. circinatus) leaveswas investigated against four microorganisms, Escherichia coli (ATCC ۲۵۹۲۲), Staphylococcusaureus (ATCC ۲۵۹۲۳), Pseudomonas aeruginosa (ATCC ۹۰۲۷) and Candida Albicans, using thebroth micro-dilution test.Methods : The freshly collected leaves of S. circinatus were minced and exhaustively extracted(۱۰۰ g) with ۲۰۰ milliliters of ۸۰% methanol for ۷۲ hours using the percolation method. Theextract was filtered and the solvent was evaporated to dryness at ۵۰°C under reduced pressure. Thedried extract was further concentrated in a speed vacuum.Results : The result was that the extract had moderate inhibitory activity against Escherichia coliand Candida Albicans, but no inhibitory activity in other species at ۵۰ mg/mL.Conclusion : In conclusion, methanolic extract from S. circinatus leaves would be of interest incontrolling infections with Escherichia coli and Candida Albicans.

کلیدواژه ها:

Antibiotics ، Inhibitory Activity ، Multiple Drugs Resistant ، Solenanthus circinnatus