Molecular characterization of Panton-valentine Leukocidin(PVL)-positive Staphylococcus aureus in Tehran, Iran

سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 110

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شناسه ملی سند علمی:

MEDISM23_150

تاریخ نمایه سازی: 16 مهر 1401

چکیده مقاله:

Background and Aim : Some Staphylococcus aureus strains produce Panton-Valentineleukocidin (PVL), a bicomponent poreforming toxin, which causes leukocyte lysis and tissuenecrosis. Currently, there is very limited information on the molecular epidemiology of PVLencodingS. aureus strains in Iran. This study aimed to determine genetic background of PVLpositiveS. aureus clinical strains isolated from Iranian patients.Methods : A total of ۲۸ PVL-positive S. aureus strains were detected from ۶۰۰ S. aureus isolatesbetween February ۲۰۱۶ and March ۲۰۱۹ from different hospitals in Tehran, Iran. Antimicrobialsusceptibility testing was performed according to the Clinical and Laboratory Standards Institute(CLSI) guidelines. Molecular genotyping was performed using SCCmec and accessory generegulator (agr) typing and pulsed-field gel electrophoresis (PFGE).Results : The highest resistance was observed against erythromycin (۱۶/۲۸, ۵۷.۱%). The lowestresistance was observed against linezolid (۱/۲۸, ۳.۶%). Moreover, ۱۹ (۶۷.۹%) out of ۲۸ S. aureusisolates were identified as MRSA, including CA-MRSA (۱۴/۱۹, ۷۳.۷%) and HA-MRSA (۵/۱۹,۲۶.۳%). By SCCmec typing, only three types (types III, IVa, andV) were found among ۱۹ PVLpositiveMRSA isolates. The most common type of SCCmec was type IVa (۱۰/۱۹, ۵۲.۶%). Theagr types of all ۲۸ PVL-positive S. aureus isolates, indicated that agr type I was the predominantone (۱۴/۲۸, ۵۰%). PFGE typing showed that ۲۴ isolates were clustered into A (۴ pulsotypes), B (۹pulsotypes), and C (۱۱ pulsotypes) clusters.Conclusion : A high prevalence of PVL-positive CA-MRSA strains was detected in Iran.Epidemiological studies have revealed that PVL gene is commonly carried by CA-MRSA havingSCC mec type IV. The majority of MRSA strains in the current (۵۰%) and previous studies in Irancarried SCCmec IV. In this study, the majority of the isolates (۵۰%) belonged to agr type I. Theagr typing results were consistent with the findings of previous studies in Iran and China. PFGEshowed a high degree of genetic diversity among PVL-positive S. aureus clones. This considerablediversity in PVLpositive MRSA strains could be explained by the possibility of isolating MRSAfrom different sources. Application of genotyping methods such as PFGE may provide a betterinterpretation of MRSA transmission sources.

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نویسندگان

Zahra Najafi olya

Hepatitis Research Center, School of medicine, Lorestan university of Medical Sciences, Khorramabad, Iran

Abbas Yadegar

Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Bita Bakhshi

Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University,, Tehran, Iran