Safety and immunogenicity of live attenuated IRIBA vaccine ina mouse model

Background and Aim : Brucellosis is one of the most important zoonotic diseases in livestockand humans, which is associated with abortion in animals and fever in humans, It has a highprevalence in Iran. IRIBA vaccine strain is a mutation of Brucella abortus strain ۲۳۰۸ that lacksthe lipopolysaccharide o chain. Cows vaccinated with IRIBA are protected against abortion. Theaim of this study was to evaluate the safety and immunogenicity of IRIBA vaccine, produced byRazi vaccine and serum research institute.Methods : For this purpose, according to the OIE sampling instructions, the required number ofsamples were randomly selected from three batches of IRIBA vaccine produced for reduced andfull dose of IRIBA vaccine. Evaluation of vaccine safety and immunogenicity of IRIBA wasperformed according the OIE protocol.Results : Mice vaccinated with this vaccine showed no growth of IRIBA in cultured spleens ofvaccinated mice. The condition of the spleen was also normal. Vaccine immunogenicity evaluationin vaccinated mice also showed that the vaccine tested had satisfactory results. The immuneresponse of unvaccinated mice showed that the value of Brucella in their spleen was at least ۴.۵units and in mice vaccinated with reference vaccine (RB۵۱) and RAZI vaccine (IRIBA) was lessthan ۲.۵ units. There was also no significant difference in the immunogenicity of mice vaccinatedwith the reference vaccine.Conclusion : The results of this study showed that the use of IRIBA vaccines is safe and inducesufficient immunity for protective against B. abortus.

کلیدواژه ها:

Brucellosis ، Safety ، immunogenicity ، IRIBA ، vaccines