Silibinin inhibits cell migration through downregulation of RAC۱ gene expression in highly metastatic breast cancer cell line

Backgrounds: Triple negative breast cancer is the most invasive breast cancer subtype andpossesses poor prognosis and survival. Rho GTPase family, especially Rac۱ participates in anumber of signaling events in cells with crucial roles in malignancy, migration and invasion oftumor cells. Silibinin, a flavonoid antioxidant from milk thistle has attracted attention in therecent decades for chemoprevention and chemotherapy of tumor cells. In this study, the effect ofSilibinin on the migration capacity of MDA-MB-۲۳۱ cells, a highly metastatic human breastcancer cell line was investigated by evaluation of Rac۱ expression.Materials and Methods: MTT wound healing and transwell assays were performed to evaluatethe effects of Silibinin on proliferation and migration of MDA-MB-۲۳۱ cells. In addition, theinfluence of the Silibinin on the expression of Rac۱ mRNAs was assessed by RT-PCR.Results: Results indicated significant dose-dependent inhibitory effect of Silibinin onproliferation and migration of MDA-MB-۲۳۱ cells. It significantly inhibited the expression ofRac۱ mRNA.Conclusion: In conclusion, the results demonstrated that Silibinin inhibits metastasis of theMDA-MB-۲۳۱ breast cancer cell line. The anticancer effect of Silibinin may contribute to theinhibition of metastasis by decreasing the expression level of Rac۱ gene. Therefore, Silibinin canbe used as an experimental therapeutic for the management of TNBC metastatic cancer, with thepromise shown in terms of increasing cancer patient survival.

کلیدواژه ها:

Silibinin ، Breast cancer ، TNBC ، Rac۱ ، Metastasis