Key genes associated with the progression of non-small cell lung adenocarcinoma in smokers and non-smokers
محل انتشار: کنفرانس بین المللی ژنتیک و ژنومیکس انسانی
سال انتشار: 1400
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 105
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شناسه ملی سند علمی:
CHGGE01_268
تاریخ نمایه سازی: 13 مهر 1401
چکیده مقاله:
Backgrounds: Non-small cell lung cancer is the most prevalent type of lung cancer, accountingfor ۸۵ percent of all cases. It is frequently diagnosed at an advanced stage and has a poorprognosis. Understanding the key genes involved in adenocarcinoma tumor progression insmokers and non-smokers can lead to early identification and multimodal treatments.Materials and Methods: GSE۵۰۰۸۱ CEL files were normalized using robust multi-arrayaverage (RMA) via the R programming language, and differentially expressed genes (DEGs) ofadenocarcinoma stages ۱ and ۲ were detected in two separate groups of smokers and nonsmokersusing the following criteria: |𝑙𝑜𝑔۲𝐹𝐶| > ۱ and P-value <۰.۰۵. The STRING databasewas used to create a PPI network, and then examined using Cytoscape to track hub genes.Enrichment study was carried using “Enrichr”.Results: In non-smokers and smokers, ۳۵۸ DEGs (۵۷ up-regulated and ۳۰۱ down-regulated) and۲۴۵ DEGs (۴۳ up-regulated and ۲۰۲ down-regulated) were identified, respectively. KEGGpathway analysis was enriched in protein digestion and absorption for smokers andphosphatidylinositol signaling system for non-smokers. According to Gene Ontology, Smokers’DEGs enriched significantly in alveolar lamellar body and non-smokers in bone morphogenesis.In smokers, three hub genes were found: CF transmembrane conductance regulator (CFTR),microtubule-associated protein ۲ (MAP۲), and NEDD۴ like E۳ ubiquitin-protein ligase(NEDD۴L). Non-smokers were found to have erb-b۲ receptor tyrosine kinase ۴ (ERBB۴) andheat shock protein ۹۰ alpha family class B member ۱ (HSP۹۰AB۱) as hub genes, which could beemployed as therapeutic targets.Conclusion: In summary, identifying discrete hub genes for both smokers and non-smokers andunderstanding the pathways and ontologies involved can help us better understand the diseaseprogression and provide novel biomarkers for future therapeutics.
کلیدواژه ها:
نویسندگان
Kimya Fazel Nezhad
Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran
Arash Bagherabadi
Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran
Saeid Latifi-Navid
Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran