Network-based Prediction of microRNAs targeting RdRp and N coronavirus-۲ genes

Backgrounds: Severe acute respiratory syndrome coronavirus ۲ (SARS-CoV-۲) is responsiblefor the ۲۰۱۹ Epidemic (COVID-۱۹). Coronaviruses are relatively large enveloped, positivesense,single-stranded RNA viruses. Experimental evidence suggests that microRNAs canmediate an intracellular defense mechanism against viral genome. Most biological processes areregulated by microRNAs, which have emerged as promising therapeutic candidates for severaldiseases. Due to the corona epidemic and high mortality, a new treatment strategy is essential.Here, we report the miRNAs of human genome that can target RdRp and N genes and suppressestheir translation.Materials and Methods: Sequences of RdRp and N genes were first obtained from the NCBIresources. Then, miRNAs were studied and predicted using online tools including miRBase,Targetscan, RNA۲۲, Cytoscape, and Metascape.Results: Regarding the scores and E-Values, it was concluded that hsa-miR-۳۶۹۱-۳p, hsa-miR-۸۰۶۶ and hsa-miR-۱۳۰۷-۳p have maximum tendency to target RdRp, N and ۳'UTR ofcoronavirus genome, respectively.Conclusion: Given the potential suppressive effect of these microRNAs, it may be useful toprevent virus replication by employing these molecules as therapy, whether by synthetic siRNAsor by increasing their expression in the body.Further research on the identification of targeted microRNA against the SARS-COV-۲ genomeand understanding the importance of microRNA as a cellular defense mechanism againstpathogenic coronavirus infections will be useful.

کلیدواژه ها:

RdRp ، N ، microRNA ، SARS-COV-۲ ، Coronavirus