Expression profile evaluation of Interferon gamma and leptin genes in infertile women compared with fertile individuals

Backgrounds: Infertility is a reproductive system disease defined by the failure to achieve apregnancy after ۱۲ months of regular unprotected sexual intercourse. One cause of femaleinfertility is LEP and Interferon-gamma malfunction. The leptin's main synthesis location andfunction is fat tissue and to control the body fat reserve, respectively. In addition, it is a naturalcontroller of appetite and transmits the brain signals indicating that the body is ready forreproduction. Another gene involved in infertility is the interferon-gamma, located onchromosome ۱۲. Interferon-gamma is a double-stranded protein produced by Th۱, CD + ۸tnatural killer cells. In this study, we investigated the expression of these two genes in infertileand fertile women.Materials and Methods: RNA was extracted from blood samples of fertile and infertile women,and then cDNA was synthesized. These genes expression was evaluated in infertile and fertilewomen using primers designed with the Real-Time PCR analysis.Results: Interferon-gamma and leptin genes have lower expression patterns in infertile womencompared with fertile individuals.Conclusion: This study results showed a significant relationship between leptin and interferongammagenes expression and infertility.

کلیدواژه ها:

Interferon-gamma ، Leptin ، Obesity ، Infertility