Effect of nuclear factor erythroid ۲-related factor silencing on the MiaPac۲ pancreatic cancer cells

Backgrounds: Pancreatic cancer (PC) is a deadly malignancy that is more common in men andelderly people (۴۰ to ۸۵ years old) and has an aggressive course. Its frequency has beengradually increasing in recent years. It accounts for ۲% of all cancers and ۵% of cancer deaths.Deregulation of Nuclear factor erythroid ۲-related factor (NRF۲) is proposed to play a keypathogenic role in pancreatic cancer. In this research, the effect of Nrf۲ knockdown wasinvestigated using specific siRNA in a pancreatic cancer cell line.Materials and Methods: First, several pancreatic cell lines were screened for high expression ofNrf۲, and MiaPac۲ was selected for further investigations. The cells were grown in a ۱۰ % RPMImedium to reach ۷۰ % confluence transfected with Nrf۲ siRNA. Total RNA was extracted,converted to cDNA and the expression level of Nrf۲ was measured by qRT PC MTT assay, andannexin V-FITC/PI staining tests were examined used for cell viability and apoptosis analysis,respectively. MTT assay and annexin V-FITC/PI staining tests were used for cell viability andapoptosis analysis, respectively.Results: According to our findings, Nrf۲ siRNA transfection had significant effects on tumorcells. Nrf۲ expression level in MiaPac۲ cells was suppressed by siRNA silencing, resulting indecreased cell viability and increased apoptosis.Conclusion: Nrf۲ silencing might be considered as a potential approach for pancreatic cancertreatment.

کلیدواژه ها:

PC ، NRF۲ ، siRNA ، knockdown ، apoptosis