Evaluation of miR-۳۸۲ expression change in the prefrontal cortex of the brain following alcoholism in male Wistar rats

Backgrounds: Alcohol has various side effects and its abuse causes severe metabolic andphysiological effects in all body systems. Given that different area of the brain, including theprefrontal cortex, play a role in causing addiction; this study aimed to investigate the geneticexpression of miR-۳۸۲ in the prefrontal cortex of the brain in rats.Materials and Methods: Fifty male Wistar rats weighing ۲۰۰ to ۲۵۰ g were prepared. Adultmice were kept under hygienic conditions at room temperature with free access to water andfood for ۱۲ hours in a light /dark period. Alcohol was administered to animals orally for ۲۱ daysat a dose of ۵ mg/kg. The mouse's head was removed and then the skull was split and theprefrontal cortex was removed. The expression of miR-۳۸۲ relative to the reference gene wasexamined using real-time PCR in this region. Statistical analysis was performed using SPSSsoftware.Results: The results showed that following the consumption of alcohol for ۲۱ days, theexpression level of miR-۳۸۲ in the prefrontal cortex significantly decreased (P <۰.۰۵).Conclusion: The study of miRNAs as mediators of molecular mechanisms that also affect theexpression of other genes can increase our understanding of the mechanism of complications ofproblems such as alcoholism.

کلیدواژه ها:

miR-۳۸۲ ، prefrontal cortex ، Alcoholism ، Male Wistar rat