Evaluation of miR-382 expression change in the prefrontal cortex of the brain following alcoholism in male Wistar rats

Backgrounds: Alcohol has various side effects and its abuse causes severe metabolic andphysiological effects in all body systems. Given that different area of the brain, including theprefrontal cortex, play a role in causing addiction; this study aimed to investigate the geneticexpression of miR-382 in the prefrontal cortex of the brain in rats.Materials and Methods: Fifty male Wistar rats weighing 200 to 250 g were prepared. Adultmice were kept under hygienic conditions at room temperature with free access to water andfood for 12 hours in a light /dark period. Alcohol was administered to animals orally for 21 daysat a dose of 5 mg/kg. The mouse's head was removed and then the skull was split and theprefrontal cortex was removed. The expression of miR-382 relative to the reference gene wasexamined using real-time PCR in this region. Statistical analysis was performed using SPSSsoftware.Results: The results showed that following the consumption of alcohol for 21 days, theexpression level of miR-382 in the prefrontal cortex significantly decreased (P <0.05).Conclusion: The study of miRNAs as mediators of molecular mechanisms that also affect theexpression of other genes can increase our understanding of the mechanism of complications ofproblems such as alcoholism.

کلیدواژه های Evaluation of miR-382 expression change in the prefrontal cortex of the brain following alcoholism in male Wistar rats:

miR-382 ، prefrontal cortex ، Alcoholism ، Male Wistar rat