The effect of cigarette smoking on epithelial proliferation in gingival inflammation

Introduction: Cigarette smoking have a destructive effect on gingival tissue. A direct relation between smoking and cancers of the oral cavity has been shown. Uncontrolled cell proliferation has a key role in cancer progression. The aim was to evaluate the impact of cigarette smoking on epithelial proliferation in gingival inflammation using ki-۶۷ marker.Method: Paraffin embedded blocks of gingival tissue with severe gingivitis in grade ۱-۳ of CPITN system from ۱۴ smokers and ۱۳ non-smokers were stained immunohistochemically for ki-۶۷ expression. The ki-۶۷ labelling index was determined in both groups and compared. T-test and Pearson correlation coefficient tests were employed at the P-Value≤۰.۰۱ probability level for data analyzing.Results: The mean of ki-۶۷ labeling index was ۴۴.۳۸±۳۰.۱ and ۴۴.۶±۲۶.۶ in smokers and non-smokers, respectively. The ki-۶۷ labeling index was not significantly different between smokers and non-smokers (P-Value=۰.۹). The intensity of gingivitis in both nonsmokers (r=۰.۰۹, P-Value=۰.۷۶) andsmokers (r=۰.۱, P-Value=۰.۵۷) were not correlated to ki-۶۷ labeling index.Conclusion: Cigarette smoke had not drastic effect on epithelial proliferation.The finding shows that the chemical ingredients of cigarette reduced the inflammation of the gum.The hyperplastic pattern of gingival epithelium was due to inflammatory process of subjacent connective tissue.

کلیدواژه ها:

Antigen Ki۶۷ ، Cigarette Smoking ، Cell ، Epithelial