The Effects of Aerobic Training and Omega-۳ Intake on Aβ۴۲, Neprilysin and γ- Secretase in the Hippocampus of Male Rats Alzheimer s model

Alzheimer disease (AD) is characterized by excessive deposition of the amyloid-β peptide (Aβ) in the central nervous system and reducing its level is the goal of many medications. Thus the aim of this study was to investigate the effect of aerobic training and omega-۳ intake on Aβ۴۲, Neprilysin and γ- secretase in the hippocampus of male Rats Alzheimer s model. ۵۰ male Wistar rats (۱۲weeks-old and weight ۲۲۲.۳۱±۱۱.۹۱ g), divided into ۵ groups including, control Alzheimer s (AC), Alzheimer s with omega ۳ intake (AO), Alzheimer training (AT), Alzheimer s with omega-۳ intake and Alzheimer training (AOT) and Healthy Control (HC). Alzheimer s disease induced by injection of homocysteine (۶۰mM) into the rat brain ventricle. Training with a speed of ۲۰ m/min (۶۰ minutes and ۵ day/week on the treadmill) was applied. The supplement group, was received Omega-۳ supplement ۸۰۰mg/kg of body weight, daily for eight weeks. Level of Aβ۴۲, Neprilysin and γ- secretase protein measured using ELISA method. In data analysis, a one-way analysis of variance and Tukey test as a post hoc, were used (P<۰.۰۵). The obtained results showed that the level of Aβ۴۲ in hippocampus of AC was significantly higher than HC group (P=۰.۰۰۱). Also the level of the Aβ۴۲ in hippocampus of AC was significantly higher than AO, AT and AOT groups (P values ۰.۰۰۱, ۰.۰۰۷ and ۰.۰۰۳ respectively). Also the γ- Secretase level in hippocampus of AC was significantly higher than HC group (P=۰.۰۰۱). Also the γ- secretase levels in hippocampus of AC group was significantly higher than AO, AT and AOT groups (P values, ۰.۰۰۲, ۰.۰۰۱ and ۰.۰۰۱ respectively). There was no significant difference between Neprilysin level of the hippocampus of research groups (P = ۰.۵۳۴). it appears that exercise training and omega-۳ consemption, can prevent amyloidgenic pathways by reducing the level of γ- secretase, and lead to reducing the level of Aβ hippocampus of Alzheimer s disease subjects. In total, aerobic exercise training and omega-۳ intake can be studied as a complementary therapy in Alzheimer s patients.

کلیدواژه ها:

Aerobic training ، Omega-۳ Intake ، amyloid-β۴۲ ، Neprilysin ، γ- Secretase ، Alzheimer's disease