Effect of aerobic training on left ventricular apoptotic and antioxidant indices in high fat diet and streptozotocin induced diabetic rats

Diabetic cardiomyopathy is one of the most common complications of type ۲ diabetes, which is usually associated with changes in the structure and function of the myocardium. On the other hand, exercise has been suggested as a potential non-pharmacological strategy for cardiomyopathy, although the effectiveness of exercise in preventing or reversing the progression of cardiomyopathy is controversial. Therefore, the aim of this study was to evaluate the effect of aerobic exercise on left ventricular apoptotic and antioxidant indices in high fat diet and streptozotocin induced diabetic rats.For this purpose, ۲۴ male Wistar rats were randomly divided into two groups of normal diet (n = ۸, ND) and high fat diet (n = ۱۶, HFD). High-fat diet rats were given a free diet containing ۵۵% fat for ۴ weeks. To diabetes induction, ۳۰ mg of streptozotocin (STZ) was injected intraperitoneally into obese rats. Diabetic rats were subdivided into two groups: control diabetic (n= ۸, CD) and diabetic-training group (n = ۸, TD). The training group performed aerobic exercises on a treadmill for ۴ weeks with an intensity of ۶۵ to ۸۰% of the maximum speed. Weight, glucose, insulin and HOMA IR were measured before and after intervention. Catalase and caspase-۹ levels were measured by ELISA and P۵۳ levels were measured by Western blotting.Statistical analysis showed that HFD and STZ induced diabetes caused hyperglycemia, increased HOMA-IR, decreased catalase activity, and increased left ventricular caspase-۹ and P۵۳. The results also showed that aerobic training increased the maximum oxygen consumption rate by ۳۷%. The TD group had lower HOMA-IR and plasma glucose than the CD group. On the other hand, aerobic training increases Catalase and decreases caspase-۹ and P۵۳.In general, the results showed that diabetic cardiomyopathy reduces the antioxidant index of Catalase and promoted proapoptotic proteins. On the other hand, ۴ weeks of increasing aerobic exercise increases Catalase activity and reduced apoptotic indices through hypoglycemic effects and insulin resistance amelioration. Therefore, aerobic training can be used as an effective non-pharmacological method in the treatment of diabetic cardiomyopathy.

کلیدواژه ها:

Aerobic training ، Apoptotic ، Antioxidant ، Diabetic ، Streptozotocin