Investigation and Evaluation of Electrochemical Behavior of Glutamic Acid Using Glassy Carbon Electrode

This study aims at the electrochemical characterization of a novel sensor for determination of the glutamic acid on the surface of a glassy carbon electrode in the presence of hydroquinone as a probe was investigated using cyclic voltammetry and differential pulse voltammetry techniques. The electrochemical behavior of hydroquinone in the presence of an amino acid on the surface of a glassy carbon electrode indicates that hydroquinone is converted to quinone during a two electron/proton reaction. The amino acid and quinone was react in michael addition reaction. We are probably facing an EC reaction mechanism. This sensor had two linear ranges from ۰.۰۱۲-۰.۱۶۸ pM and ۰.۱۶۸-۱.۲۸ nM with a detection limit of ۰.۴۷۸ pM for glutamic acid.Indirect voltammetry is a sensitive, fast and accurate method that was used in this study.Glutamic acid have a special role in improving the function of the central nervous system by participating in the metabolism of carbohydrate brain proteins and accelerating and facilitating the oxidation process [۱]. Glutamic acid has carboxylic acid groups in its side chains [۲]. Due to the importance of measuring amino acids in several analytical methods have been used to measure amino acids, biological and pharmaceutical compounds [۳, ۴].Linear dynamic range was obtained over the range ۰.۰۱۲-۰.۱۶۸ pM and ۰.۱۶۸-۱.۲۸ nM of glutamic acid and the limit of detection was ۰.۴۷۸ pM based on S/N=۳ by differential pulse voltammetry.