Integrated Bioinformatics Analysis of Hub Genes and Pathways in Thyroid Cancer

Thyroid cancer (TC) is the sixth most common type of cancer among women worldwide.The aim ofthepresent study was to identify hub genes and pathways in Thyroid cancer by microarray expression profilingFurthermore,a protein-protein interaction(PPI) network was constructed, and ۸ hub genes were identifiedbased on this network.Microarray data selected for bioinformatic analysis is Gene Expression data storedwith GSE۳۵۵۷۰, GSE۳۶۷۸, GSE۳۳۶۳۰ code in the National Center for Biotechnology Information (NCBI)Gene Expression Omnibus (GEO) database. Thyroid cancer and healthy control groups were compared,Thatincluding ۱۰۰ thyroid tumors and ۷۴ normal tissues to obtain the intersection of differentially expressedgenes, and a protein-protein interaction network was constructed to obtain the HUB gene and analyzed bySearch Tool for the Retrieval of Interacting Genes online tool and R software Based on the whole network,we identified ۸ hub genes that included CCNA۲,FEN۱,CCNB۱,CDK۱,RRM۲,CDC۴۵,CHRDL۱,UBE۲C.which were highly expressed in TC tissues. Bioanformatic data suggested that the expression levels of CDK۱,UBE۲C and CCNA۲ genes were also upregulated in other histological subtypes of thyroid carcinoma. Highexpression of FEN۱,CDC۴۵,CCNB۱,CHRDL۱ and RRM۲ gene significantly decreased disease-free survivalof patients with other thyroid carcinomas ,and Enrichment analysis showed that these hub genes wereprimarily accumulated in ‘cell cycle’ and ‘p۵۳ signaling pathway’, ‘viral carcinogenesis’These findingssuggest that may several hub genes( CCNA۲, FEN۱,CCNB۱,CDK۱,RRM۲,CDC۴۵,CHRDL۱,UBE۲C) andpathways, which will contribute to elucidating the pathogenesis of TC and providing therapeutic targets forTC.

کلیدواژه ها:

Thyroid cancer ، Hub Genes ، Bioinformatics analysis