Relative expression analysis of COL۱A۱ mRNA in oral tongue squamous cell carcinoma: a study based on gene expression omnibus (GEO) and bioinformatics analysis

Oral Tongue Squamous Cell Carcinomas (OTSCC) is one of the most aggressive and common malignanttumor of the head and neck distinct. Our study aimed to define a competitive endogenous RNA (CeRNA)network that provides valuable biomarkers and boosts the treatment process of OTSCC. Therefore,bioinformatics analysis targets novel biomarkers for diagnosis and curing OTSCC. From the NCBI GeneExpression Omnibus (GEO) (http://www.ncbi.nlm.nih.gov/geo/), we obtained the gene expression data of ۱۰OTSCC tumor and control samples (GSE۱۶۰۰۴۲). GEO۲R analyzed the data sets to find out differentiallyexpressed genes in patients (DEGs). A threshold value of |logFC| > ۲ and a p-value< ۰.۰۵ were chosen, andone of the most significant genes was taken to miRWalk ۲.۰ to find target miRNA. Target miRNA wassearched in LncBase v.۳, and appropriate lncRNAs were found. At last, Cytoscape software (۳.۹.۰) was usedto show the interaction between the components of the CeRNA network. Pathway enrichment was performedby KEGG. COL۱A۱ had a significant high expression in the tumor samples (logFC > ۲, adj. p. value < ۰.۰۵).Moreover, mir-۶۱۵-۳P is a novel suppressor for COL۱A۱ (score: ۱.۰۰). miR-۵۱۶-۳p have RNA interactionswith, NORAD, XIST, and MIAT lncRNAs. COL۱A۱ is an invasion-related gene observed in the‘extracellular matrix’ (ECM) and cell adhesion signaling pathway. It has been reported to be involved inmultiple biological behaviors, including cell proliferation, invasion, metastasis, and angiogenesis. miR-۶۱۵-۳p have a significant suppressor effect on an over-expressed gene (COL۱A۱) in OTSCC patients. Also, ithas the significant RNA interaction with NORAD, XIST, and MIAT lncRNAs. The mentioned lncRNA canregulate the OTSCC development and metastasis by affecting the expression level of COL۱A۱ by regulatingcell proliferation.

کلیدواژه ها:

Oral Tongue Squamous Cell Carcinomas (OTSCC) ، mir-۶۱۵-۳P ، COL۱A۱ ، Biomarker