Investigation of the interaction between bta-miR۲۴۱۰ and PTEN gene in dairy cattle production

Predicting microRNAs related to target genes using bioinformatics tools saves time and laboratory testingcosts. One of the most important economic traits in the dairy cattle industry is milk production, which iscontrolled by many genes and microRNAs. In this study, we investigated the interaction between btamiR۲۴۱۰and the PTEN gene. For this purpose, initially the target genes of bta-miR۲۴۱۰ were extracted frommiRWalk ۲.۰ database and according to the importance of PTEN gene in the milk production process, it wasselected from the target genes. Then, the gene sequence was downloaded from the NCBI database and themiRBase database was used to find the bta-miR۲۴۱۰ sequence. The results of interaction of bta-miR۲۴۱۰and PTEN gene with MFE = -۲۹.۹ kcal/mol show the high effect of this microRNA on PTEN gene. Theeffects of PTEN target gene on milk production have been studied in other studies, and It has been reportedthat this gene initiates lactation by playing a role in the AKT signaling pathway. The use of bioinformaticsstudies along with new techniques can be very helpful in increasing the speed and more accurateidentification of biomarkers. Finally, the results of this study showed that bta-miR۲۴۱۰ by targeting the PTENgene, which is one of the important genes involved in important biological processes of milk production, canalter milk production by altering the expression of PTEN gene in dairy cattle. Therefore, this gene can befurther studied as a biomarker in future studies.

کلیدواژه ها:

Dairy cattle ، Genetic interaction ، miRNA ، Milk production