Effects of kisspeptin incubation on the mature mouse testicular sperms quality: An experimental study

Background: Sperm quality has an important role in the success of assisted reproductive techniques, by adding some bioactive agents with a positive impact on sperms, it can be improved. Objective: This study aimed to evaluate the effects of kisspeptin on the sperm motility criteria, Lactate dehydrogenase-C (LDHC) activity, acrosomal reaction, and capacitation in the mouse testicular sperm in vitro. Materials and Methods: Sperm samples were extracted from testes of ۹۶ male Balb/C mice weighing ۲۵-۳۰ gr, aged ۶-۸ wk. Then, they were separated into ۴ parts; ۲ controls and ۲ kisspeptin-treated aliquots; each one incubated for either ۱۵ or ۳۰ min. The sperm motility and the LDHC activity were evaluated, and also the frequency of the non-capacitated, intact, and acrosomal-reacted sperms were evaluated by staining with Wheat germ agglutinin, Peanut agglutinin, and Concanavalin A, respectively. The stained sperms were analyzed by flow cytometry and fluorescent microscope. Results: Our result showed that kisspeptin increased both the sperm motility (p = ۰.۰۴) and LDHC enzyme activity (p = ۰.۰۴) after ۱۵ min of incubation. At the same time, it did not impact the frequency of the non-capacitated, intact and acrosomal-reacted sperms after incubation in the same period (p = ۰.۱۶). Conclusion: A ۱۵ min period of incubation with kisspeptin could be applicable for evaluating sperm motility and LDH activity.

کلیدواژه ها:

Testis ، Spermatozoa ، Kisspeptin ، Lactate dehydrogenase ، Acrosome. ، بیضه ، اسپرم ، کیس­پپتین ، لاکتات دهیدروژناز ، آکروزوم.