Evaluation the effects of resveratrol on cyto-toxicity and reactive oxygen species production comparing with cisplatin effects in a prostate cancer cell line (LNCap)

Introduction: Prostate cancer is often caused by a mutant or by a disorder in DNA modification. Cisplatin is a potent chemical anticancer drug that causes cellular resistance; moreover, it shows side effects in normal tissues. Resveratrol is a polyphenol compound present in many plants species, including grapes, peanuts, and berries, as an antioxidant that prevents the transformation of normal cells into cancer cells, as well as the anti-cancer action in multiplying cancer cells. The aim of this study was to evaluate and compare the effects of resveratrol with cisplatin on cytotoxicity and production of reactive oxygen species in a prostate cancer cell line (LNCaP). Methods: Prostate cancer cell line (LNCaP) was purchased from the Pasteur Institute. The cultured cells were treated with concentrations of ۱, ۱۰, ۱۰۰, ۱۰۰۰ μg /ml of Res and cisplatin separately. The survival rate of cancer cells was examined using MTT assay and spectrophotometry. In addition, the production of reactive oxygen species in treated prostate cancer cell line was evaluated using flow cytometry.Results: IC۵۰ for cisplatin was ۱۲.۵۴ µg /ml and for Res it was ۲۰۵.۶ µg/ ml. The results of MTT assay showed that Res has cytotoxic effect in all examined concentration, and this effect is dose dependent. However, cisplatin showed higher cytotoxic effect comparing to Res. Moreover, Res showed strong antioxidant activity. Conclusion: Res as a plant derivative has anti-cancer effects and antioxidant properties. Regarding to the side effect of cis-platin, it can be a good candidate for using in chemotherapy. The studies on other cell lines or animal model of cancer is required to reveal more details.

کلیدواژه ها:

cancer ، cisplatin ، resveratrol ، reactive oxygen species