Histological Study of The Effect of Chitosan Scaf-folds Loaded on The Different Concentrations of Hesperi-din Flavonoid in The Rat Model of Femoral Fracture

Objective: Despite the success of bone graft in the healing of fracture, it has several limitations relating to donor-site mor-bidity, infection, bleeding, nerve damage and chronic pain. For that, the alternative biomaterials as scaffolds should be made with major influences on cell adhesion, proliferation and dif-ferentiation. Hesperidin, citrus flavonoid, inhibits bone loss by decreasing osteoclast formation and function. In this research, chitosan scaffolds with different concentrations of Hesperidin in rat femur defect repair were investigated.Materials and Methods: Thirty adult male Wistar rats weigh-ing ۲۰۰-۲۵۰ g were used and after creating fracture line on the right femur, they were divided randomly into six groups: con-trol (femoral fracture model), ۵ groups treated with chitosan scaffold with different concentrations of Hesperidin (۰, ۰.۰۱, ۰.۱, ۱ and ۱۰%). The chitosan scaffolds were prepared from solutions with chitosan (at concentration of ۱۲۰۰mg/۶۰cc ace-tic acid۱%) and evaluated wettability, porosity, degradation and compatibility by MTT, SEM and FTIR. After ۴ month, animals were sacrificed and the lesiond femurs extracted and stained with Hematoxylin and eosin (HandE) and Masson's trichrome (MT).Results: The results of MTT showed that the scaffold has no toxic effects on stromal cells. SEM and FTIR results showed the improvement of cell-scaffold interactions. Histological and CT-Scan studies showed that the defect in treated groups (۱ and ۱۰%) was fully replaced by new bone and connective tis-sue compared to control and Hesperidin (۰,۰.۰۱, ۰.۱%) treated groups. A stable collagenous matrix suitable for the osteoblastic proliferation and differentiation was formed in Hesperidin ۱۰% treated group compared to control.Conclusion: It seems that the chitosan scaffold loaded with ۱ and ۱۰% of Hesperidin has a potential role in the healing pro-cess bone lesions and it can be suitable as a therapeutic strategy in tissue engineering.

کلیدواژه ها:

Tissue Engineering ، Scaffold ، Chitosan ، Hesperidin ، Fracture