D۲-۴۰ A Helpful Marker in Assessment of Lymphatic Vessel Invasion in Carcinoma of BreastTumor characteristic Number of cases (percentage %)

Background & Objective: Breast cancer is the most common malignancy in Iranian women and worldwide. Lymphatic vessel invasion (LVI) was found to be an independent prognostic factor in many carcinomas, including invasive carcinoma of the breast. The aim of this study was to compare the hematoxylin and eosin (H&E) staining method and the use of the immunohistochemistry (IHC) marker, D۲-۴۰, for evaluating LVI in breast carcinoma specimens. Materials & Methods: The study was conducted on ۵۰ patients undergone surgery between the years ۲۰۱۰ and ۲۰۱۵ in Rohani Hospital, Babol, Iran with invasive carcinoma of the breast with Census sampling method. LVI was assessed using H&E staining and two IHC markers, i.e., D۲-۴۰ and CD۳۱, by two surgical pathologists.  Results: LVI was detected in ۲۵ (۵۰%) patients by H&E and in ۱۴ (۲۸%) patients by D۲-۴۰. Twelve out of ۲۵ patients with positive LVI in H&E were confirmed by D۲-۴۰ and ۲ out of ۲۵ patients with negative lymphatic vessel in H&E. Only one case showed weak staining of CD۳۱ proving LVI. These results showed a significant difference between the H&E staining and D۲-۴۰ IHC study for LVI detection (p =۰.۰۰۴). Conclusion: The D۲-۴۰ IHC marker is helpful in the diagnosis and confirmation of LVI in invasive carcinoma of the breast. CD۳۱ is not suitable for the evaluation of lymphatic vessels.

کلیدواژه ها:

Breast ، Carcinoma ، Monoclonal antibody D۲-۴۰