۸-Hydroxydaidzein suppresses breast cancer stem cells to cease breast cancer progression

  • سال انتشار: 1400
  • محل انتشار: پنجمین کنگره بین المللی سرطان
  • کد COI اختصاصی: CANCERMED05_056
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 174
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نویسندگان

Sadegh Rajabi

Traditional Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Shokoofe Noori

Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

چکیده

Introduction: Cancer stem cells (CSCs) have a key role in different stages of cancer development and therapy resistance. ۸-Hydroxydaidzein (۸-OHD) is known as an anti-cancer agent against many tumors. Accordingly, the aim of this study is to evaluate the effects of ۸-OHD on the suppression of breast cancer stem-like cells (BCSCs). Methods: The anti-proliferative and pro-apoptotic properties of ۸-OHD were examined by MTS assay and flowcytometry. The expression levels of stemness markers and JAK۲/STAT۳ proteins were measured by quantitative real time-PCR (qRT-PCR) and western blotting, respectively. Results: ۸-OHD significantly decreased three out of six stemness markers and remarkably reduced viability and induced apoptosis of spheroidal and parental cells compared to controls. Further experiments using CD۹۵L, as a death ligand, and ZB۴ antibody, as an extrinsic apoptotic pathway blocker, showed that ۸-OHD induced apoptosis through the intrinsic pathway, proposing a mechanism by which ۸-OHD triggers apoptosis. Results of western blot analysis also revealed a marked decline in the phosphorylation of JAK۲ and STAT۳ proteins. Conclusion: Our data unraveled the anti-BCSC activity of ۸-OHD by reducing stemness markers, inducing toxicity, and triggering apoptosis of these cells and parental cells. Our results also proposed a novel mechanism by which ۸-OHD stimulated apoptosis of BCSCs.

کلیدواژه ها

Breast cancer therapy, JAK۲, STAT۳, Apoptosis, Resistance

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