Structural characteristics of a damaged DNA

Ionizing radiation and cellula r metabolism can cause oxidative DNA damage. The reccurrence of dam aging, if no t be repaired by DNA repair enzymes, may alte r the genetic information and can lead to cancer or aging. I n this respect, the most abundant oxidative damage, ۸-ox o-۷,۸-dihyd ro-۲’-deoxy guanosine ( ۸-oxoguanine), has rece ived a great attention. Guanine, due to its low oxidative potential, has a tendency to be oxidiz ed in C۸ position. The produced ۸-oxoG has the ability to form both ۸-oxoG:C basepair a nd ۸-oxoG: A Hoogsteen mispair, which produces GC to TA transversion muta tion. Here we used molecular dynamics, to study struct ural and dynamics differences betwe en a native DNA and damaged DNA containing two ۸-oxoG lesions in opposite strands.

کلیدواژه ها:

۸-oxoguanine ، D NA damage ، Molecular dynamics ، GROMACS