Frequency of class I and II integrons in the clinical isolates of Pseudomonas aeruginosa with multidrug resistance in Shahrekord teaching hospitals and Isfahan Shahid Chamran hospital during ۲۰۱۶-۲۰۱۷

Background and aims: Increasing the prevalence of nosocomial infections by multidrug resistant (MDR) Pseudomonas aeruginosa hasseverely challenged the choice of treatment and led to an increased mortality rate. Thus, this study investigated the frequency of class I andII integrons and its association with MDR.Materials and Methods: A total of ۱۷۵ P. aeruginosa isolates were collected from Shahrekord teaching hospitals and Isfahan ShahidChamran hospital during ۱۲ months (from April ۲۰۰۸ to March ۲۰۰۹). Antibiotic susceptibility was determined by disc diffusion accordingto the Clinical and Laboratory Standards Institute (CLSI) Antimicrobial Susceptibility Testing. The E-test strips of imipenem, ciprofloxacin,and amikacin were used to identify the minimum inhibitory concentration and MDR bacteria. Finally, the frequency of class I and IIintegrons genes was evaluated by using the polymerase chain reaction test.Results: The highest antibiotic resistance and the highest susceptibility belonged to meropenem (۸۶.۹%) and polymyxin B (۹۶.۰%) by discdiffusion, respectively. By the E-test, the highest and lowest resistance rates were reported for imipenem (۹۷.۲%) and ciprofloxacin (۸۶.۸%),respectively. The frequency of MDR strains was ۸۲.۳% as well. The frequency of class I and II integrons was ۵۷.۷% and ۱۷.۷% in all P.aeruginosa isolates, as well as ۶۸.۱%and ۲۱.۵% in the MDR isolates, respectively. There was also a significant correlation between I andII integrons and MDR.Conclusion: Overall, the resistance to different antibiotics and the frequency of MDR strains among the studied P. aeruginosa isolateswere very high. There was also a significant correlation between integrons and multidrug resistance. Regarding the role of integrons inthe transfer of drug-resistant genes and the development of MDR strains, the use of appropriate diet and accurate determination of thesusceptibility pattern of P. aeruginosa isolates are considered necessary.

کلیدواژه ها:

Integron ، Pseudomonas aeruginosa ، Multidrug resistance