The effects of all trans retinoic acid, vitamin D۳ and their combination on plasma levels of miRNA-۱۲۵a-۵p, miRNA-۳۴a, and miRNA-۱۲۶in an experimental model of diabetes

Objective: The purpose of this study was to evaluate the effects of ATRA (all trans retinoic acid), vitamin D۳, and their combination on circulating levels of miR (MicroRNA) -۱۲۵a-۵p, miR-۱۲۶, and miR-۳۴ain diabetic rats.Materials and Methods: Total miRNA was extracted from plasma samples. miRNA expression profiles of ۳۰ rats in five groups were analyzed after ۴-week intervention. The expression levels of miRNAs were measured using qRT-PCR.Results: We analyzed the expression of miR-۱۲۶, miR-۱۲۵a-۵p, and miR-۳۴a in serum among all five groups (p=۰.۲۶۸). The levels of miRNA-۱۲۶ (p=۰.۰۰۴) and miR-۱۲۵a-۵p (p=۰.۰۱۴) showed a significant difference among our experimental groups. The circulating levels of miR-۱۲۶ decreased in DC (Diabetic control) group compared to the HC (Healthy control) group (p=۰.۰۰۹). In addition, vitamin D۳+ATRA supplementation increased miR-۱۲۶ expression (p=۰.۰۱۴). Moreover, the levels of miR-۱۲۵a-۵p decreased in the DC group compared to the HC group (p=۰.۰۱۹).Conclusion: The expression of miR-۱۲۶ and miR-۱۲۵a-۵p decreased in diabetic rats. Also, vitamin D۳+ATRA can be considered a new therapeutic agent that can elevate miR-۱۲۶ expression and prevent diabetes-related cardiovascular complications.

کلیدواژه ها:

Plasma miRNAs ، miR-۱۲۶ ، Type ۲ diabetes ، Cardiovascular Disease