Increasing the caspase ۳/۷ activity in MDA-MB-۲۳۱ breast cancer cell line by the methanolic extract of Prangos crossoptera

Breast cancer is regarded as a multifactorial disease that leads to many deaths among women. Detrimental side effects, drug resistance, and a high rate of mortality have prompted the need to discover novel, safe, and effective drugs. Medicinal plants as an enormous source of native components are one of the main candidates for discovering new anticancer drugs. Prangos genus is one of the important medicinal plants with valuable therapeutic virtues and anticancer properties of several Prangos species have been reported. This study aimed to evaluate the anticancer properties of the methanolic extract of Prangos crossoptera in the MDA-MB-۲۳۱ cell line.In the current study, MDA-MB-۲۳۱ (human breast adenocarcinoma) and HDF (Human Dermal Fibroblasts) cell lines were treated with different concentrations of methanolic extracts of Prangos crossoptera (۰, ۵۰, ۱۵۰, ۲۵۰, ۳۵۰, and ۴۵۰ μg/mL) for ۲۴, ۴۸, and ۷۲ h. Cytotoxicity effects of the samples on the MDA-MB-۲۳۱ and HDF cells were examined using [۳-(۴, ۵-dimethylthiazolyl)-۲, ۵-diphenyl-tetrazolium bromide (MTT) method. Also, caspase ۳/۷ activity was measured using caspase ۳/۷ colorimetric assay kit to investigate the effects of the extracts on the programed cell death pathway in the treated cells.The results of the current study revealed that growth of MDA-MB-۲۳۱ cells significantly inhibited after ۴۸ and ۷۲h of treatment with extract compared to the control group (p < ۰.۰۵). Furthermore, measurement of caspase ۳/۷ activity showed that methanolic extract of Prangos crossoptera significantly increased the caspase ۳/۷ activity in treated cells compared to the control group.Prangos crossoptera seems to affect the programed cell death pathway by increasing the caspase ۳/۷ activity in MDA-MB-۲۳۱ cell line.

کلیدواژه ها:

MDA-MB-۲۳۱ ، Medicinal plants ، Prangos crossoptera ، Caspase ۳/۷