Evaluation of Nostoc commune extract effects on locomotor activity ۶-OHDA-induced in model of Parkinson’s disease

Emerging evidence continues to demonstrate that increased oxidative stress in the pathology of Parkinson’s disease (PD) Causes irreversible loss of dopaminergic neurons. PD is held responsible for the characteristic motor signs and symptoms (such as tremor, postural instability, bradykinesia, hypokinesia, rigidity). Cyanobacterium Nostoc commune (NC) has been traditionally used as a healthy food and medicine for centuries, especially that exhibit strong antimicrobial and antioxidant activities. The present study aims to evaluate the effects of the NC extract on motor dysfunction ۶-OHDA-induced in animal model of Parkinson's disease.Method & material In this experimental study, ۴۰ Wistar male rats (۲۰۰–۲۵۰ g) were divide the following groups: i) control, ii) ۶-OHDA-lesioned, iii) ۶-OHDA infused supplemented with ۵۰ mg/kg NC extract (۶-OHDA+NC ۵۰), and iv) ۶-OHDA infused supplemented with ۱۰۰ mg/kg NC extract (۶-OHDA+NC ۱۰۰). The Parkinson's model was induced by ۶-OHDA (۱۰ μg in ۲ μl/site) injection unilaterally (AP: +۱ mm; L: +۲.۵ mm; D: +۴.۵ mm). After ۲۴ h, intrastriatal ۶-OHDA-lesioned rats received the NC extract (۵۰ and ۱۰۰ mg/kg) orally for three weeks. The open field test (OFT) on day ۲۱ was carried out.Result The results showed that injection of the ۶-OHDA-lesioned group decreased locomotor activity in the OFT (p < ۰.۰۰۱) compared to control group. NC ۵۰ and NC ۱۰۰ treatment increased the locomotor activity into the OFT (p < ۰.۰۰۱) compared with the lesioned group. Besides, NCE ۱۰۰ showed better therapeutic effects than NCE ۵۰.Discussion In conclusion, Regular treatment with NC extract in rats for three weeks significantly decreased the locomotor activities. Therefore, it can be stated that neuroprotective action in improvement of motor dysfunction may be attributed to antioxidant activities. Thus, using these substances may be useful by modulating the level of antioxidant activity and protecting the nervous system against ۶-OHDA toxicity as a pharmacological tool.

کلیدواژه ها:

Nostoc commune ، locomotor activity ، Parkinson’s disease ، ۶-hydroxydopamine