Improving the growth and biochemical traits of black cumin (Nigella sativa L.) by applying Humic acid and Azotobacter

The safe production (chemical-free) of medicinal and aromatic plants has been the focus of interest of various researchers worldwide. Therefore, the aim of this greenhouse experiment was to study the effect of Humic acid and Azotobacter on growth, seed yield and oil content and constituents of black cumin (Nigella sativa L.). The treatments included Control (Plants were not received any bio-fertilizers), Humic acid, Azotobacter (Azotobacter chroococcum) and combination of Humic acid and Azotobacter. The results clearly showed that combination use of Humic acid and Azotobacter positively affected plant morphological and biochemical traits including, plant height (۳۸ cm), capsule number per plant (۱۴.۸۳), seed yield per plant (۷.۳ gr), essential oil percentage (۰.۴۱ %) and Fixed oil percentage (۲۷.۶ %). Moreover, the GC-MS analysis of essential oil indicated that the main constituents including Thymoquinone (۳۱.۵۲ %), p-cymene (۷.۳۲ %), Limonene (۷.۱۵ %) and Thymol (۳.۸ %), were also affected by Humic acid +Azotobacter application.

کلیدواژه ها:

Essential Oil ، Fixed Oil ، Thymol ، trans-Anethole