Evaluation of the protective effect of green tea polyphenols for sub-acute toxicity of diazinon in mice

Background: Studies have shown that an organophosphorus pesticide such as diazinon induces oxidative stress injury and damage in various tissues. Objectives: This study conducted to investigate the effects of green tea polyphenols (GTP) on the liver function of diazinon -poisoned animals. Methods: Twenty-eight mice were divided into four groups of the control, GTP (۴۰۰ mg/kg), diazinon + GTP, and diazinon. Animals received malathion ۸۰ mg/kg and SA ۴۰۰ mg/kg for one week through intraperitoneal injection. Then, their liver and blood samples were extracted, and alanine aminotransferase, aspartate aminotransferase concentrations in serum as well as biomarkers of oxidative stress such as Total Antioxidant Capacity (TAC), Lipid Peroxidation (LPO) and catalase (CAT) activity in the liver tissue were measured. Results: The results indicated that the GTP administration decreased the level of liver LPO compared with that in the diazinon group. Also, receiving GTP increased liver TAC and CAT levels in mice, which this difference was significant than the diazinon group. In addition to, the GTP group showed a significant reduced in liver enzyme levels than the diazinon treated group. Conclusion: Based to the results, GTP exerted protective effects against diazinon poisoning, by decrease of oxidative stress. Thus, GTP can be an antioxidant to counteract the risk effects of diazinon poising in liver tissue

کلیدواژه ها:

Diazinon ، Green tea polyphenols (GTP) ، Oxidative stress ، Mice