Influence of different strains of Agrobacterium rhizogenes, culture medium, age and type of explant on hairy root induction in Echinacea angustifolia

Hairy roots culture is a potential system for secondary metabolite production in medicinal plants. In this research, hairy root induction in Echinacea angustifolia was established using Agrobacterium rhizogenes. The effects of dif-ferent A. rhizogenes strains (AR۱۵۸۳۴, A۴, K۵۹۹ and MSU۴۴۰) in two solid growth regula-tor-free culture media, half strength MS salts with B۵ vitamins and WPM, were evaluated on hairy root induction in ۶۰-day-old explants. The highest percentage of root induction was ob-tained by AR۱۵۸۳۴ strain in the WPM medium (۴۶.۶%). Also, the effects of age (۲۰, ۴۰ and ۶۰ day-old) and type (leaf, leaf lamina and petiole) of explant on hairy root induction were investi-gated by AR۱۵۸۳۴ strain in the WPM medium. The high frequency of hairy root induction was obtained in leaf explants (۵۲.۴%) and ۴۰ day-old leaf explants (۶۲.۳%).Transgenic hairy roots were confirmed by PCR analysis with specific primers of rolB gene.

کلیدواژه ها:

Agrobacterium rhizogenes ، Echinacea angustifolia ، Hairy roots ، Explant