Beneficial effect of symbiotic supplementation during pregnancy in high fat diet-induced metabolic disorder in rats:Role of Chemerin

Objective: Metabolic disorders during pregnancy have detrimental effect on mother and her fetus. Symbiotic supplementation can improve metabolic disorders. The aim of this study was to assess the impact of symbiotic supplementation on the levels of Chemerin, lipid profile, glucose, and insulin resistance in pregnant rats fed a high-fat diet (HFD).Materials and methods: Thirty female Wistar rats (weighing ۲۰۰–۲۲۰ g) were randomly assigned into three groups: control group, HFD group, and HFD + symbiotic group. The control group received the regular diet (fed lab chow); the HFD group received HFD alone; and the HFD + symbiotic group that in addition to taking HFD, was gavaged daily during pregnancy with a symbiotic solution, which contained Lactobacillus rhamnosus and Bacillus coagulans at a concentration of ۱۰۸ CFU/ml and fructooligosaccharide ۱۰%. On the ۱۰th day of the experiment, the animals became pregnant. At the end of pregnancy period, birth weight of offspring, levels of blood glucose, total cholesterol, triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), insulin, and chemerin level were measured.Results: Birth weight of offspring in the HFD + symbiotic group was significantly lower than in the HFD group (P < ۰.۰۲). Similarly, insulin, insulin resistance, TG, total cholesterol, and LDL levels in the HFD + symbiotic group were significantly lower than in the HFD group (all P < ۰.۰۱). Symbiotic supplementation significantly reduced chemerin concentration in HFD + symbiotic group than HFD group (P < ۰.۰۰۸). Chemerin levels had a significant and positive correlation with food intake in the first ten days of the experiment and gestation period (P < ۰.۰۱, P < ۰.۰۴), fasting blood sugar (FBS) (P < ۰.۰۱), Total cholesterol (P < ۰.۰۵) and homeostatic model assessment (HOMA) index (P < ۰.۰۱). Moreover, chemerin levels had a significant and negative correlation with HDL (P < ۰.۰۱) and insulin (P < ۰.۰۲) levels.Conclusion: Based on our results, symbiotic supplementation has beneficial effects on metabolic disorders and improves weight gain during pregnancy, pup birth weight, FBS, insulin resistance and lipid profile. These advantages of symbiotic supplementation could be mediated by change in chemerin secretion.

کلیدواژه ها:

Chemerin ، Symbiotic ، Metabolic syndrome ، Pregnancy ، High-fat diet