Nuclear factor erythroid-۲ related factor ۲ overexpressed mesenchymal stem cells transplantation, improves renal function, decreases injuries markers and increases repair markers in glycerol-induced Acute kidney injury rats

Objective(s):Recently cell therapy is a promising therapeutic modality for many types of disease including acute kidney injury (AKI). Due to the unique biological properties, mesenchymal stem cells (MSCs) are attractive cells in this regard. This study aims to transplant MSCs equipped with nuclear factor E۲-related factor ۲ (Nrf۲) in rat experimental models of acute kidney and evaluate regeneration potential of injured kidney especially expression of injury and repaired biomarkers. Materials and methods:Nrf۲ was overexpressed in bone marrow-derived MSCs by pcDNA.۳.۱ plasmid. AKI was induced using glycerol in rat models. The regenerative potential of Nrf۲-overexpressed MSCs was evaluated in AKI-Induced animal models using biochemical and histological methods after transplantation. Expression of repaired genes, AQP۱ and CK-۱۸, as well as injury markers, Kim-۱ and Cystatin C, was also assayed in engrafted kidney sections. Results:Our results revealed that transplantation of Nrf۲-overexpressed MSCs into AKI-induced rats decreased blood urea nitrogen and creatinine and ameliorated kidney regeneration throughout ۱۴ days. Upregulation of repaired markers and downregulation of injury markers were considerable ۱۴ days after transplantation.  Conclusions: Overexpression of Nrf۲ in MSCs suggests a new strategy to increase efficiency of MSC-based cell therapy in AKI.

کلیدواژه ها:

Acute kidney injury ، AQP۱ ، KIM-۱ ، Mesenchymal stem cells ، Nrf۲