Development of ۱۵۳Sm-DTPA-SPION as a theranostic dual contrast agents in SPECT/MRI

Objective(s): This study describes the preparation, biodistribution of ۱۵۳Sm-DTPA-SPION after intravenous injection in rats. Materials and Methods: The chelator DTPA dianhydride was conjugated to SPION using a small modification of the well-known cyclic anhydride method. Conjugation was done at a ۱: ۴ (SPION: ccDTPA) molar ratio. Conjugation reaction was purified with magnetic assorting column (MACs) using high gradient magnetic field following incubation, the radio labeled conjugate was checked using RTLC method for labeling and purity checked. Results: The RTLC showed that labeling yield was above ۹۹% after purification and the compound have good in vitro stabilities until ۴۸ hr post injection in the presence of human serum. The biodistribution of ۱۵۳Sm-DTPA-SPION in rats showed dramatic uptake in the reticuloendothelial system (RES) and their clearance is so fast in other organs especially in the blood. Biodistribution results show that after ۳۰ min post injection more than ۸۴% of injected activities were taken up by the liver and spleen (about ۶۴% and ۲۰%, respectively). Conclusion: Due to magnificent uptakes of this radiotracer in the liver and spleen and their fast clearance from other tissues, especially in blood, it is suggested that this radiotracer would be a potential candidate for RES theranostic purposes.

کلیدواژه ها:

Biodistribution ، Liver ، Multi-modality imaging ، Super paramagnetic iron - oxide nanoparticles ، Theranostic ، ۱۵۳Sm