Cxcr۴ expression is associated with time–course permanent and temporary myocardial infarction in rats

Objective(s): Experimental myocardial infarction triggers secretion of Stromal cell-derived factor۱ and lead to increase in the expression of its receptor "CXCR۴" on the surface of various cells. The aim of this study was to evaluate the expression pattern of CXCR۴ in peripheral blood cells following time-course permanent and temporary ischemia in rats. Materials and Methods: Fourteen male Wistar rats were divided into two groups of seven and were placed under permanent and transient ischemia. Peripheral blood mononuclear cells were isolated at different time points, RNAs extracted and applied to qRT-PCR analysis of the CXCR۴ gene.  Results: Based on repeated measures analysis of variance, the differences in the expression levels of the gene in each of the groups were statistically significant over time (the effect of time) (P-value< ۰.۰۰۱). Additionally, the difference in gene expression between the two groups was statistically significant (the effect of group), such that at all times, the expression levels of the gene were significantly higher in the permanent ischemia than in the transient ischemia group (P-value<۰.۰۰۱). Moreover, the interactive effect of time-group on gene expression was statistically significant (P-value<۰.۰۰۱). Conclusion: CXCR۴ is modulated in an induced ischemia context implying a possible association with myocardial infarction. Checking of CXCR۴ expression in the ischemic changes shows that damage to the heart tissue trigger the secretion of inflammatory chemokine SDF, Followed by it CXCR۴ expression in blood cells. These observations suggest that changes in the expression of CXCR۴ may be considered a valuable marker for monitoring myocardial infarction.

کلیدواژه ها:

CXCR۴ gene expression ، Time-course ، Permanent cardiac ischemia ، Temporary cardiac ischemia