Curcumin enhances liver SIRT۳ expression in the rat model of cirrhosis

Objective(s): Bill duct ligation (BDL) is a representative model of biliary cholestasis in animals. Curcumin has a protective effect on the liver; however, its underlying mechanisms are not completely known. This study explored the hepatoprotective activity of curcumin on hepatic damage via measuring the expression of sirtuin۳ (SIRT۳), AMP-activated protein kinase (AMPK), carnitine palmitoyltransferase ۱A (CPT-۱A), isocitrate dehydrogenase۲ (IDH۲) and manganese superoxide dismutase (MnSOD) as well as the level of serum lipid profile in the BDL fibrotic rat model. Materials and Methods: The study consisted of four groups (n=۸ for each of Wistar rats): sham group, sham+curcumin (sham+Cur) group (received curcumin ۱۰۰ mg/kg/day), BDL+Cur group, and BDL group. Transcription levels of SIRT۳, AMPK, CPT-۱A, IDH۲, MnSOD and protein expression level of SIRT۳ were measured by real-time PCR and Western blotting, respectively. Results: It was identified that SIRT۳, AMPK, CPT-۱A, IDH۲ and MnSOD expression significantly decreased in BDL rats compared to sham rats; however, in the curcumin treatment of BDL rats, the expression of these factors increased significantly compared to BDL (P-value <۰.۰۵). It was, moreover, observed that treatment of BDL rats with curcumin reduced liver injury as verified by a reduction in the levels of total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL) and increase in high-density lipoprotein (HDL)(P-value <۰.۰۵). Conclusion: Curcumin reduced liver damage and oxidative stress in the liver tissue of BDL rats through up-regulation of SIRT۳, AMPK, CPT-۱A, IDH۲ and MnSOD as well as changing the level of serum lipid profile.

کلیدواژه ها:

Curcumin ، Gene expression ، Liver Cirrhosis ، Rats ، SIRT۳ protein