A Novel Nano-sensing Assay for Determination of Food Pathogenic Agent Based on Bimetallic Metal Organic Frameworks (MOFs) for determination of S. typhimurium in Food Samples

Monitoring of pathogenic bacteria plays a vital role in precluding foodborne disease outbreaks. In this research work, a genosensor based on innovative label-free DNA was developed for the detection of Salmonella. typhimurium (S.typhimurium). To realize this objective, bimetallic MOF is synthesized and mixed with AuNPs which are then stabilized on the Au electrode surface. Electrochemical and Impedimetric techniques were used for exploring the applicability of the fabricated genosensor. Under optimal circumstances, LOD and LOQ have acquired at ۰.۰۷ pM and ۰.۲۱ pM. Great selectivity against one, two, and three-base mismatched sequences was also shown for fabricated biosensing assay. Furthermore, negative genosensing assay control for investigating selectivity was provided by the ss-tDNAs of H. Influenzae and Shigella dysenteriae bacteria. Well-fabricated genosensing bio-assay represents better performance, great specificity, high sensitivity, increased active sites, and finally results in an increase in the electron transfer rate. It is to be noted that the organized genosensing bio-assay is capable of being re-used and re-generated in a straightforward manner to estimate the hybridization process

کلیدواژه ها:

Salmonella typhimurium ، Genosensor ، DNA hybridization ، bimetallic Fe/Mn MOFs ، AuNPs ، multi-walled carbon nanotubes