Isolation and Identification of Staphylococcus saprophyticus Lytic bacteriophages from Urban and Hospital Wastewaters in Gorgan city

Background and Aim : Staphylococcus saprophyticus is the ability to cause urinary tract infections. The introduction of the first lytic phage of S.saprophyticus by researchers at Golestan University of Medical Sciences raised the possibility of identifying its variants for complementary therapies in urinary tract infections. This study aimed to isolate and identify S.saprophyticus lytic bacteriophages from the hospital and municipal effluent samples in Gorgan.Methods : S.saprophyticus were collected from medical diagnostic laboratories in Gorgan diagnosis of bacteria can be performed with microbiological tests as well as PCR of the ۱۶SrRNA gene. Antibiotic resistance of isolates was performed according to CLSI s.saprophyticus bacteriophages were isolated using spot test and Double-Layer Agar (DLA) method. After purification, the host domain and morphological characteristics of bacteriophages were examined by electron microscopy.Results : ۳۵ isolates with an initial diagnosis of S.saprophyticus were collected in Golestan province, all of which were confirmed as S.saprophyticus after resuscitation and purification by biochemical tests and ۱۶ SrRNA confirmation tests. The results of the antibiogram test showed that all isolates were sensitive to nitrofurantoin, linezolid, gentamicin antibiotic discs. Out of ۶۴ effluent samples, ۵ bacteriophages were isolated from municipal effluents. bacteriophages were named VB_SsapS-۱۰۱, VB_Ssaps۱۰۵-, VB_SsapS-۴۶, VB_SsapS-۱۱۱, VB_SsapM-۱۹۰ according to the Kropinsky method. VB_SsapS ۱۱۱(۶۰%) and VB_SsapS۱۰۵ (۵۱.۴%) showed the highest host range. None of the ۵ bacteriophages had an inhibitory effect on other bacteria. Conclusion : S.saprophyticus showed good sensitivity to existing antibiotics, for the treatment of urinary tract infections Due to the wide host range of the two bacteriophages VB_SsapS۱۱۱- and -VB_SsapS۱۰۵, they can be a good candidate for adjuvant therapy.

کلیدواژه ها:

Staphylococcus saprophyticus ، bacteriophage ، urinary tract infection ، antibiotic susceptibility ، sewage