Detection of hybrid uropathogenic/diarrheagenic Escherichia coli strains among uropathogenic E. coli isolates

Background and Aim : Urinary tract infection) UTI) is one of the most common bacterial infections that caused by Escherichia coli at least in ۸۰% of cases. There is evidence that some uropathogenic E. coli (UPEC) isolates have diarrheagenic source; therefore, in the present study we investigated the presence of diarrheagenic markers among UPEC isolates. Methods : A total of ۲۰۰ UPEC isolates were screened by multiplex -PCR for the presence of main diarrheagenic marker genes including aggR and pCVD for enteroaggregative E. coli, invF gene for the enteroinvasive E. coli, stx۱, stx۲, eaeA and hlyA for enterohemorrhagic E. coli, stla, stlb and lt for enterotoxigenic E. coli and escV for enteropathogenic E. coli.Results : aggR, pCVD, eaeA genes were found in ۲۰, ۱۸, and ۱ isolates, respectively, while the other genes were not found. Therefore, ۲% of UPEC strains were hybrid EAEC/UPEC. Conclusion : Results indicated that some diarrhea-causing E. coli isolates may also have the potential to cause urinary tract infections. Among these, the EAEC pathotype is more important than the other diarrheagenic E. coli pathotypes.

کلیدواژه ها:

Urinary tract infection ، Escherichia coli ، diarrheagenic marker ، uropathogenic E. coli