Phyco-fabrication of bimetallic nanoparticles (zinc–selenium) using aqueous extract of Gracila riacorticata and its biological activity potential otentials

Background and Aim : Zinc selenium nanoparticles (ZnSe NPs) are widely used in many industrial, environmental and biomedical fields. However, the biological potential of ZnSe NPs has not been studied in detail. The present study focused on ZnSe NPs algae-based synthesis using aqueous extract of seaweed, Gracilaria corticata Methods : Successfully synthesized ZnSeNPs were characterized by analytical methods such as UV–Vis, FTIR, XRD, SEM, TEM, DLS, Zeta potential and EDX analyses; additionally, the biological function of ZnSe NPs was performed.Results : The ZnSe NPs exhibited a certain absorbance peak at ۳۵۰–۴۰۰ nm in the UV–vis spectrum. The FTIR showed the possible functional groups associated with biomolecules involved the ZnSe NPs synthesis. SEM and TEM demonstrated that ZnSe NPs were spherical in shape with size range of ۵۰–۲۵۰. XRD and EDX showed NPs crystallite size of ۵۵.۵ nm and their elemental composition that constitute selenium and zinc (۱:۱.۵ ratio). These NPs showed antioxidant activity about ۶۷% and antibacterial activity against broad spectrum of bacterial strains. Biofilm inhibition by ZnSe NPs was occurred on P. aeruginosa and B. subtilis at ۵۰ and ۴۰ ?g/ml. Anticancer activity against HTB-۹ (ATCC ۵۶۳۷) and KB (ATCC CCL-۱۷) with IC۵۰ of ۱۹.۲۴ and ۲۸.۴۲ ?g/ml.Conclusion : In conclusion, the green synthesis of ZnSe NPs can provide the requisite criteria for therapeutic and preventive applications.

کلیدواژه ها:

Zinc selenide nanoparticles (ZnSe) ، Green synthesis ، Gracilaria corticata ، Biological activity