In vitro Antibacterial Activity and Phytochemical Screening of Hydroalcohlic Extract of Seeds of Polylophium involucratum (Pall). Boiss. from Ramsar - Iran

Background and Aim : Natural products have been a major source of new drugs. Plant used for traditional medicine contains a wide range of substances that can be used to treat chronic as well as infectious diseases. Polylophium involucratum (Pall). Boiss. is a medicinally essential plant used for the treatment of diverse infectious diseases. The aim of this project was to investigate the antibacterial effect of hydroalcohlic extract of seeds of Polylophium involucratum (Pall). Boiss. (Apiaceae) from Ramsar - Iran, to the three species of common pathogenic bacteria resistant to multiple drugs in vitro such as:, Staphylococcus aureus (PTCC ۱۱۱۳), Bacillus subtilis (PTCC ۱۱۵۶) and Escherichia coli (PTCC ۱۳۹۹).Methods : In this research, hydroalcohlic extract of seeds of Polylophium involucratum (Pall). Boiss. prepared by microwave assisted extraction (MAE) method. The antibacterial activity was determined by (Disk Diffusion Method) against three strains of Gram-positive and Gram-negative bacteria. Also, phytochemical study was performed by using standard phytochemical methods. Results : The hydroalcohlic extract of seeds of Polylophium involucratum (Pall). Boiss. exhibited good antibacterial potential against gram positive and gram negative bacterial strains. Also, our preliminary phytochemical analysis of seeds extract using hydroalcohlic as solvent confirmed the presence of (Flavonoids, Terpenoids, Coumarins, Phenols, Cardiac glycosides, Quinones and Saponins). This indicates that antibacterial activities may be due to presence of secondary metabolites. Conclusion : The findings of the present study demonstrated the potential of phytochemicals from Polylophium involucratum (Pall). Boiss. seeds, a natural source, in the pathway of developing a novel antibacterial agent able of treating bacterial infections.

کلیدواژه ها:

Antibacterial drugs ، Polylophium involucratum (Pall). Boiss. ، Bacillus subtilis ، Terpenoids.